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STED (access provided through the Peter O'Donnell Jr. Brain Institute)

STED SR
 

This system is owned by the Peter O'Donnell Jr. Brain Institute. The Abberior Facility Line system is a cutting-edge, high-end 3-D STED super-resolution imaging system capable of providing 40 nm isotropic resolution in samples up to 70 µm thick - xy down to 25nm.

  • Adaptive Illumination (RESCue, DyMIN and MINFIELD) provides less phototoxicity and drastically reduces photobleaching.
  • Adaptive Optics System with Deformable Mirror makes super-resolution imaging possible in deep tissues. 
  • Objectives: 60X (NA1.42 WD 0.15mm) oil; 60X (NA1.20 WD 0.28mm) water;  60X (NA1.30 WD 0.30 mm) silicon.
  • Lasers: 405 nm (confocal), 485 nm, 561 nm, 640 nm pulsed lasers (STED and confocal). 595 nm and 775 nm STED depletion STED lasers.
  • Stage top incubator: OKO Lab Uno premixed gas