Genomic DNA Extraction
AutoGen FlexSTAR
The Autogen FlexSTAR is a laboratory workstation designed to provide automated nucleic acid purification from large sample volumes for high-throughput research applications. It can process up to 30 samples in 2.5 hours while providing complete sample tracking. We routinely obtain ~500 µL of archive-quality genomic DNA from 1-5 mL whole blood, buffy coats, 1-4 mL Oragene saliva, and more.
Among the many features of the Autogen FlexSTAR are consistent sample identification and tracking, a robotically accessible centrifuge, automated mixing, weighing, pouring and tube-to-tube transfer. In addition, specially designed processing tube are easily capped, uncapped, and transported throughout the instrument using a combination of robotic arms and transfer racks.
Blood Tubes
Blood samples in purple top EDTA tubes should be kept at 4oC. Blood from multiple tubes are combined to obtain a buffy coat from which genomic DNA is isolated. The DNA pellet is rehydrated with 500 µL of DNA hydration solution. Only one tube of DNA will be returned for each order of genomic DNA.
- For samples less than 10 mL of whole blood, the entire sample will be processed by hand. The DNA pellet will be rehydrated in 250 µL of DNA hydration solution.
Current Protocol for DNA Purification from less than 10 mL of whole blood
Buccal Swab
Buccal swabs should be stored at room temperature. The DNA pellet will be rehydrated in 50 µL of DNA hydration solution.
Current Protocol for DNA Purification from Buccal Samples
Oragene Spit Kit
Oragene Spit Kit samples should be stored at room temperature; they are stable for up to two years. The DNA pellet will be rehydrated in 400 µL of DNA hydration solution. For more information on Oragene products, please visit www.dnagenotek.com or contact anusha.korecki@dnagenotek.com.
- Only 1 aliquot of DNA is processed and stored per sample. All DNA will be stored at 4oC (short-term) or -20oC (long-term).
Current Protocol for DNA Purification from Oragene Saliva Samples