Resources

Vitrobot Training Materials

• Vitrobot Protocol
• Sample preparation slides
• Training resources on Vitrobot operation and grid preparation

EM Operation Protocols

• Protocol of using Multi Records in SerialEM
• Protocol of using Hole Finder in SerialEM
• SerialEM setup on Krios
• Krios FEG direct alignment
• Krios C2 aperture direct alignment
• Pixel size and dose information
• SerialEM setup on Arctica
• Arctica alignment

SPA Data Processing

• crYOLO Protocol
• TOPAZ Protocol (Relion 3.1)

Protein Handling

• Millard, et al. (2003) - High-throughput protein expression Solubility
• Bondos & Bicknell; (2002). Aggregation
• Hammarstrom, et al.; (2001). Rapid screening for improved solubility
• Lindwall, et al.; (2000). A sparse-matrix approach to solubilization
• Woestenenk, et al.; (2003). Screening methods for biophysical properties
• Bagbi; et al.; (2001). Optimizing solubility
• Hoffmann, et al.; (2005). Assessing foldedness using NMR Concentration Determination
• Pace, et al.; (1995). How to determine an extinction coefficient

Robotic Handling at ESRF (by J. L. Ferrer)

Presentation (PDF)

Videos

Note: there is no sound on these videos.