Resources

Microscopy course

Online videos of our course Optical Microscopy for Biomedical Research": 2005-2006, 2018

Reference Library

• Microscopy Basics
  • Microscopy Primer
  • ImageJ User Guide
• Imaging Do's and Don'ts
  • The good, the bad and the ugly (Nature)
  • What's in a picture? The temptation of image manipulation (JCB)
  • Seeing is believing? A beginner's guide to practical pitfalls in image acquisition (JCB)
  • Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images (Science and Engineering Ethics)
  • Tutorial: guidance for quantitative confocal microscopy (Nature Protocols)
• Fluorescent Proteins
  • A guide to choosing fluorescent proteins (Nature Methods)
  • Interactive visualization of fluorescent protein properties (UCSF)
  • Interactive photoswitchable protein properties (UCSF)
• Quantum Dots
  • Potentials and pitfalls of fluorescent quantum dots for biological imaging (Trends in Cell Biology)
  • Advances in fluorescence imaging with quantum dot probes (Science)
• Colocalization of Fluorophores
  • A practical guide to evaluating colocalization in biological microscopy (Science and Engineering Ethics)
  • A guided tour into subcellular colocalization analysis in light microscopy (Wiley Online Library)
  • Quantitative Fluorescence Co-localization to Study Protein–Receptor Complexes (Methods in Molecular Biology)
• FRET (Förster resonance energy transfer)
  • Fanciful FRET (Science Signalling)
  • Cerulean, Venus, and VenusY67C FRET Reference Standards (Biophysical Journal)
  • Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy (Nature Protocols)
• TIRF (Total internal reflection fluorescence microscopy)
  • Total internal reflection fluorescence microscopy: technical innovations and novel applications (Current Opinion in Biotechnology)
  • Overcoming Optical Challenges to Live-Cell TIRF Microscopy (Photonics Media)
• Super-Resolution Imaging
  • A New Wave of Cellular Imaging (Annual Review of Cell and Developmental Biology)
  • Navigating challenges in the application of superresolution microscopy (Journal of Cell Biology)
  • Structured Illumination (Nature Protocols)

Training Videos

Please watch these excellent training videos for the Zeiss LSM880, produced by Jason Kirk, director of the OiVM:

• Introduction to confocal microscopy 
• Basic operations of the LSM880 (start watching at 2:20min)
• Operating the AiryScan on the LSM880