Flow Cytometry Core
The University Flow and Mass Cytometry Facility is a shared resource facility providing cell analysis and sorting to the entire UTSW campus. The facility offers training of investigators, consultation of experimental design, data acquisition, data analysis, cell sorting, and data management. The facility is equipped with eight benchtop analyzers for user operated data acquisition and four sorters which are staff operated. Two mass cytometers and an imaging mass cytometer are also available with facility staff operation. All services are provided through online iLabs accounts, and individual users may reserve instruments and acquire training through their individual accounts.
David Farrar, Ph.D.
Equipment
| Instrument | Laser Wavelength(s) (nm) | Colors | Hourly Fee |
|---|---|---|---|
| Calibur | 488, 635 | 4 | $42.26 |
| Canto | 488, 561, 635 | 8 | $55 |
| LSR | 488, 532, 405, 635 | 16 | $75.65 |
| Aurora | $75.65 | ||
| ImageStream | 488, 634 | 5 | $75.65 |
| CellStream | 488, 561, 405, 640 | 16 | $55 |
| Bioreader | Elispot reader | $22 |
There are four cell sorters available in the facility; facility staff operates the MoFlo and three Arias. The sorters are equipped with various combinations of two to five lasers with up to 18 colors available. Both sterile sorting and biohazardous containment are available with the Aria sorter.
| MoFlo and Arias | Up to 18 colors | $103.10 for first hour |
Mass Cytometry
Mass cytometry is available on up to 50 simultaneous markers with metal conjugated antibodies. The use of metal isotopes eliminates autofluorescence issues and decreases the marker crosstalk and need for crosstalk subtraction. Mass cytometry can be utilized for both samples in solution, Helios, or with tissue slides, Hyperion.
| Cytof – Helios | Up to 50 markers | $202.40 |
| Cytof – Hyperion | Up to 40 markers | $270 |
Antibody Bank
The core has built an antibody bank of common antibodies to both mouse and human which are conjugated to metal isotopes for use on the Cytofs. The bank is constantly expanding to meet the needs of researchers. Please email for an updated list. Antibodies, isotope barcodes, and staining reagents may be ordered through iLabs.
Training
Mandatory training by facility staff is required for all users. After training users are granted access to run the benchtop analyzers and utilize analysis computers. Training is $100 for the Scan and Caliburs and $150 for the Canto, CyAn, LSR II, ImageStream, and CellStream. Training is performed with samples from the first experiment, which is included in the training fee.
Data Analysis
Computers and multiple software packages are available for data analysis free of charge to users.