Services
Allelic Discrimination Assays
Allelic Discrimination (AD) assay is a multiplexed, end-point assay that detects variants of a single nucleic acid sequence. The presence of two primer/probe pairs in each reaction allows genotyping of the two possible variants at the single-nucleic polymorphism (SNP) site in a target template sequence.
For each sample in an AD assay, a unique pair of fluorescent dye detectors is used. One fluorescent dye detector is a perfect match to the wild type (allele 1) and the other fluorescent dye detector is a perfect match to the mutation (allele 2).
The Allelic Discrimination assay classifies unknown samples as Homozygotes or Heterozygotes .
You can order your specific probes from Life Technologies® by visiting their Real-Time PCR Assays product page.
Generally we will need 10ul of 10-20ng/ul DNA in Strip-Ease tubes along with the probes you would like to use. We ask that all new clients either call or email Ross Wilson to discuss your project’s details.
GenePrint (Human Only)
The GenePrint® 10 System allows co-amplification and three-color detection of nine human loci, including the ASN-0002 loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818) as well as D21S11.
These loci collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109. Used for cell line authentication.
Complies with ANSI/ATCC authentication guidelines.
We will need 10ul of 10-20ng DNA in Strip Tubes.
We ask that all new clients either call or email Ross Wilson to discuss your project’s details.
Microsatellite Marker Analysis
Microsatellite analysis includes PCR amplification of the microsatellite loci using fluorescently labeled primers; labeled PCR products are then analyzed by capillary electrophoresis (CE) or electrophoresis to separate the alleles by size. We have more than 800 markers to choose from and will be happy to discuss them in more detail with you.
We will need 10ul/marker of 30-50ng/ul DNA in Strip tubes.
We ask that all new clients either call or email Ross Wilson to discuss your project’s details.