Genetic identification of novel regulators of necroptosis

Another direction of my research program is to identify novel molecular regulators of necroptosis. To that end, we engineered a haploid cell line that will undergo induced-necroptosis, resulting in almost 100% cell demise. We then did genome-wide CRISPR-Cas9 knockout screen and identified a list of genes that are required for necroptosis. Many of these genes previously have not been implicated in necroptosis regulation. Their function are now being thoroughly examined.  

Whole genome CRISPR-Cas9 knockout screen