We use a variety of analytical approaches to uncover the complex structure-function relationship among intrinsically disordered proteins. One of our major tools is cross-linking mass spectroscopy (XL-MS), which defines contact points within a protein and provides vital clues about its architecture. We also employ structural analysis (e.g., NMR, x-ray crystallography, or cryo-EM), computational methods of protein-structure prediction, and biochemical methods (e.g., binding assays).
This hybrid structural analysis led to a recent major finding, that structural changes in tau underlie whether or not it aggregates into pathogenic amyloid fibrils. Initial XL-MS results provided structural information that constrained feasible structures using the Rosetta method of structure prediction. Kinetic analysis further refined the models.
Chen, D, et al. (2019) Tau local structure shields an amyloid-forming motif and controls aggregation propensity. Nat Comm 10, 2493.
Mirbaha H, et al (2018). Inert and seed-competent tau monomers suggest structural origins of aggregation. eLife:e36584.